Effect of Substrate Concentration on Catalase :: Papers

Effect of Subst prise Concentration on Catalase Aims This is an experiment to break down how the concentration of the substrate Hydrogen bleach (H2O2) affects the rate of reception of the enzyme Catalase. reason Information Enzymes much(prenominal) as Catalase are protein molecules, which are found in living cells. They are used to speed up specific reaction within the cell. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen bleach from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into irrigate and oxygen as shown in the equations below. Formula It is able to speed up the decomposition of Hydrogen peroxide because the process of its active site matches the shape of the Hydrogen peroxide molecule. This type of reaction where a molecule is small down into smaller pieces is called an Anabolic Reaction. Apparatus Need For The Experiment. 1. 250 cm3 tripe Beakers 2. field glass Thistle Funnel 3. Graduated Measuring Cylinder 4. Cork Borer 5. 250 cm3 Glass Cylinders. 6. Digital Stop clock 7. Scalpel 8. Tap and Distilled Water 9. Plastic rule. 10. guard Goggles. Method To test out how the concentration of Hydrogen Peroxide affects the rate of reaction first set up the apparatus and prepare the divergent concentrations of Hydrogen peroxide as below Concentration of Hydrogen Peroxide Volume of Hydrogen Peroxide (cm3) Volume of Tap Water (cm3) 25 vol 250 0 20 vol 200 50 15 vol 150 ampere-second 10 vol 100 150 5 vol 50 200 1. utilise the cork borer, take a sample of potato, cut this piece in cm intervals using the scalpel and plastic rule. Then cut these a gain ground 2 sections, so there is now 5 sections per 1cm piece of potato.